The ladies are the wives and relatives of political prisoners.
There were swarms of foreign press running after them as they walked in silence about three blocks.
They held pictures of political prisoners including the arrest of a Lady in White and her husband who have been jailed for three months.
"We are here...while political prisoners exist, there will be Ladies in White visiting Saint Rita Church praying for freedom for Cuba, freedom for political prisoners, and for human rights ... and we will walk in the streets of Cuba for the rights of these men," said Berta Soler, the head of the Ladies in White.
Soler preached to reporters about their fight for change in Cuba as police officers watched on from a distance.
She explains she wants just one minute with Pope Benedict XVI in hopes of sharing her concerns about the people of Cuba.
The meeting, like today's march, is a risk. The women risk their lives every time they march in unison.
But it's a risk she said she is always willing to take.
"The love for our family, the love for our country, is stronger than a jail cell."
These women have such love for their country, a strong desire to see it at its highest potential.
And with that, the women entered the church for Sunday mass and headed out to march.
Two uniformed lines...the women holding gladiolas...silence.
Only the hustle of the reporters chasing after them could be heard, and the honks from passing cars opposing the media coverage.